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The 2nd response cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis/NIR. As drawn in Fig - https://www.artstation.com/julieanndesalorenz1/profile. 9, the reaction may likewise include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As displayed in Fig. 9, this might be because the H4B radical acts as an electron sink in a later point in the reaction, so as to allow NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.